ary013  (R&D Systems)

Journal: Bioactive Materials

Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration

doi: 10.1016/j.bioactmat.2024.08.032

Figure Lengend Snippet: Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). Error bars: mean ± SD; n = 2 (Fig. E, number of wells) or 3 (the others, number of mice); * p < 0.05, ** p < 0.01, **** p < 0.0001, two-way ANOVA.

Article Snippet: Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E).

Techniques: Expressing, Fluorescence, Imaging, Western Blot, Stable Transfection, Microarray

Journal: Disease models & mechanisms

Article Title: High-fat diet induces C-reactive protein secretion, promoting lung adenocarcinoma via immune microenvironment modulation.

doi: 10.1242/dmm.050360

Figure Lengend Snippet: Fig. 4. Identification of cytokines and adipokines potentially contributing to HFD-induced lung cancer progression. Mouse lung tissue was used to profile a total of 62 cytokines, and the serum was used to profile a total of 38 adipokines through antibody arrays. IPA software was used to further analyze the results on regulatory network prediction and the correlation of clinical outcomes through KM plotter analysis. (A) Left panel: a cytokine antibody array was prepared using lung cancer tissues from LC-HFD and LC-RD mice. The red and blue boxes represent upregulated and downregulated cytokines, respectively, in the LC-HFD compared with LC-RD group. Right panel: quantification of cytokines using ImageJ software. (B) Left panel: the levels of 38 different adipokines in mouse sera. The yellow boxes represent CRP upregulated in the LC-HFD group compared with that in the LC-RD group. Right panel: quantification of adipokines using ImageJ software. (C) Establishing the potential regulatory network underlying cytokine and adipokine modulation in HFD- treated mice with lung cancer using IPA software. (D) Quantitative real-time PCR analysis of Crp mRNA expression levels in the liver and adipose tissues. (E) Prognostic prediction power for correlation between progression-free survival and the cytokines and adipokines mentioned in A and B. The survival data were analyzed by KM plotter analysis combined with multivariate Cox proportional hazards regression analysis. Patients were grouped according to auto- selection of the best cut-off. *P<0.05; ***P<0.001. BAT, brown adipose tissue; CRP, C-reactive protein; eWAT, epididymal white adipose tissue; HR, hazard ratio; iWAT, inguinal white adipose tissue.

Article Snippet: A Proteome ProfilerTM Array Mouse Adipokine Array Kit (R&D Systems, ARY013) was used to detect 38 different adipokines in mouse serums.

Techniques: Software, Ab Array, Real-time Polymerase Chain Reaction, Expressing, Selection